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Designations:
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Neuro-2a
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Depositors:
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RJ Klebe
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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adherent
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Organism:
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Mus musculus (mouse)
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Morphology:
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neuronal and amoeboid stem cells
Neuro-2a细胞，小鼠脑神经瘤细胞
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Source:
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Strain: A
Organ: brain
Disease: neuroblastoma
Cell Type: neuroblast;
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Cellular Products:
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acetylcholinesterase
tubulin
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Neuro-2a细胞，小鼠脑神经瘤细胞
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Applications:
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transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
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Virus Susceptibility:
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Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
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Antigen expression:
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H-2, a haplotype; Mus musculus, expressed
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Cytogenetic Analysis:
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modal number = 95; range = 59 to 193.
Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes.
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GenoType:
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albino
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Comments:
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Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine [22161].Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells.The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein [PubMed: 5263744]. The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. (see: http://www.oie.int/Eng/Normes/Mmanual/A_00044.htm)Tested and found negative for ectromelia virus (mousepox).
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Subculturing:
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Protocol:
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
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Preservation:
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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