JAR 人胎盘绒毛癌细胞的详细资料

ATCC® Number:  HTB-144™      Price:  $287.00  
Designations:  JAR  
Depositors:   RA Pattillo  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: placenta 
Disease: choriocarcinoma 
Cellular Products: estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing  
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DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 7,10 
D13S317: 11 
D16S539: 9,10 
D5S818: 10,11 
D7S820: 10,11 
THO1: 6,7 
TPOX: 8,11 
vWA: 16,18 
Cytogenetic Analysis: This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%.
Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found. 
Isoenzymes:  AK-1, 1 
ES-D, 2 
G6PD, B 
GLO-I, 1 
PGM1, 1-2 
PGM3, 1-2 
Age:  fetus  
Gender:  male  
Ethnicity:  Caucasian  
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: Twice per week
 
Preservation:  Freeze medium: Culture medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020 
References: 2156: et al., Pattillo RA. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971.