Designations: |
Panc 08.13 |
Depositors: |
EM Jaffee |
Biosafety Level: |
1 |
Shipped: |
frozen |
Medium & Serum: |
See Propagation |
Growth Properties: |
adherent |
Organism: |
Homo sapiens (human) |
Morphology: |
epithelial
|
Source: |
Organ: pancreas
Disease: adenocarcinoma |
Cellular Products: |
cytokeratins 7 and 18 [50655] |
Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
Tumorigenic: |
Yes |
Oncogene: |
K-ras + |
Antigen expression: |
MHC class I +; MHC class II - [50655] |
DNA Profile (STR): |
Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 13
D5S818: 13,14
D7S820: 11
THO1: 6,9.3
TPOX: 8,12
vWA: 18 |
Age: |
85 years |
Gender: |
male |
Ethnicity: |
White |
Comments: |
Panc 08.13 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.
The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
The cells have a reported plating efficiency of 40%. [50655] |
Propagation: |
ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%
Temperature: 37.0°C |
Subculturing: |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. |
Preservation: |
culture medium 95%; DMSO, 5% |
Doubling Time: |
20 hrs |
Related Products: |
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020 |
References: |
50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 |