| Designations: |
Panc 05.04 |
| Depositors: |
EM Jaffee |
| Biosafety Level: |
1 |
| Shipped: |
frozen |
| Medium & Serum: |
See Propagation |
| Growth Properties: |
adherent |
| Organism: |
Homo sapiens (human) |
| Morphology: |
epithelial
|
| Source: |
Organ: pancreas
Disease: adenocarcinoma |
| Cellular Products: |
cytokeratins 7 and 18 [50655] |
| Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| Isolation: |
Isolation date: May 5, 1995 |
| Tumorigenic: |
Yes |
| Oncogene: |
K-ras + |
| Antigen expression: |
MHC class I +; MHC class II - [50655]
Blood type B; Rh+ |
| Age: |
77 years adult |
| Gender: |
female |
| Ethnicity: |
White |
| Comments: |
Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.
The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
The cells have a reported plating efficiency of . [50655] |
| Propagation: |
ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
| Subculturing: |
Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add media once per week. Fluid change one to two times per week. |
| Preservation: |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Doubling Time: |
46 hrs |
| Related Products: |
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020 |
| References: |
50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 |
