| Designations: |
HT-1376 |
| Depositors: |
S Rasheed |
| Biosafety Level: |
1 |
| Shipped: |
frozen |
| Medium & Serum: |
See Propagation |
| Growth Properties: |
adherent |
| Organism: |
Homo sapiens (human) |
| Morphology: |
epithelial
|
| Source: |
Organ: urinary bladder
Tumor Stage: grade 3
Disease: carcinoma |
| Cellular Products: |
fibrinolytic activity; interferon |
| Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| Applications: |
transfection host (Roche FuGENE® Transfection Reagents) |
| Tumorigenic: |
Yes |
| Reverse Transcript: |
negative |
| DNA Profile (STR): |
Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18 |
| Cytogenetic Analysis: |
modal chromosome number = 104 to 121 |
| Isoenzymes: |
G6PD, B |
| Age: |
58 years |
| Gender: |
female |
| Ethnicity: |
Caucasian |
| Comments: |
The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar. |
| Propagation: |
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing: |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks. |
| Related Products: |
purified DNA:ATCC CRL-1472D |
| References: |
22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 |
