| Designations: |
CEM/C1 |
| Depositors: |
WG Harker |
| Biosafety Level: |
1 |
| Shipped: |
frozen |
| Medium & Serum: |
See Propagation |
| Growth Properties: |
suspension |
| Organism: |
Homo sapiens |
| Morphology: |
lymphoblast
|
| Source: |
Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast; |
| Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Age: |
4 years |
| Gender: |
female |
| Ethnicity: |
Caucasian |
| Comments: |
CEM/C1 is a camptothecin (CPT) resistant derivative of the human T cell leukemia cell line CCRF-CEM (see ATCC CCL-119).
The cell line was selected and subcloned in 1991 for resistance to CPT.
The cells exhibit cross resistance to both the water soluble (topotecan) and water insoluble (9-amino-CPT and 10,11-methylenedioxy-CPT) analogs of CPT.
CEM/C1 cells are approximately 31 fold less sensitive to CPT than the parental CEM cells.
CEM/C1 cells display atypical multidrug resistance (MDR) and altered topoisomerase I catalytic activity.
Resistance to CPT is stable for up to six months. |
| Propagation: |
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
| Subculturing: |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6. |
| Preservation: |
culture medium 95%; DMSO, 5% |
| Doubling Time: |
26 hrs |
| Related Products: |
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-119 |
| References: |
22339: Kapoor R, et al. Altered topoisomerase I expression in two subclones of human CEM leukemia selected for resistance to camptothecin. Oncol. Res. 7: 83-95, 1995. PubMed: 7579731
23117: Fujimori A, et al. Mutation at the catalytic site of topoisomerase I in CEM/C2, a human leukemia cell line resistant to camptothecin. Cancer Res. 55: 1339-1346, 1995. PubMed: 7882333 |
