COLO 320HSR 人结肠癌细胞的详细资料

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human  
Tissue  colon  
Product Format  frozen  
Morphology  rounded and refractile  
Culture Properties  mixed, adherent and suspension  
Biosafety Level  1  
Disease  Dukes' type C, colorectal adenocarcinoma  
Age  55 years  
Gender  female  
Ethnicity  Caucasian  
Storage Conditions  
Karyotype  modal number = 24; range = 49 to 61.
The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.  
Images    
Clinical Data  55 years
Caucasian
female 
Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone 
Tumorigenic  Yes  
Effects  Yes, in nude mice 
Comments  The line is negative for CEA, CSAp and colon antigen 3.
The cells are weakly positive for keratins and vimentin 
Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
 
Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. 
1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
3.Add appropriate aliquots of the cell suspension to new culture vessels.
4.Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days 
Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase 
Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 9
TPOX: 8,9
vWA: 15,18 
Isoenzymes  ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2

Name of Depositor  GE Moore  
References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 
 

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874