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Designations:
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CCRF-SB [CCRF SB]
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Depositors:
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GE Foley
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Biosafety Level:
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2 [Cells Contain HERPESVIRUS ]
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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suspension
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Organism:
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Homo sapiens (human)
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Morphology:
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lymphoblast
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Source:
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Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: B lymphoblast;
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Antigen expression:
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HLA A1, A2, B12, B17, Cw2
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DNA Profile (STR):
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Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
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Isoenzymes:
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G6PD, B
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Age:
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11.5 years
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Gender:
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male
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Ethnicity:
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Caucasian
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Comments:
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A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
Derived from a buffy coat preparation from a patient with ALL.
The cells are EBNA positive.
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Propagation:
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ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
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Subculturing:
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Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
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Related Products:
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derived from same individual:ATCC CCL-120.1
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References:
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24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 CCRF-SD 人外周血淋巴细胞CCRF-SD 人外周血淋巴细胞CCRF-SD 人外周血淋巴细胞CCRF-SD 人外周血淋巴细胞CCRF-SD 人外周血淋巴细胞
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