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产品名称: 供应 CRL-1420 MIAPaCa-2 胰腺癌细胞系
产品型号: MIAPaCa-2
品牌: 995
产品数量:
产品单价: 面议
日期: 2024-09-04

供应 CRL-1420 MIAPaCa-2 胰腺癌细胞系的详细资料

 

CRL-1420 MIAPaCa-2 胰腺癌细胞系

 
 的详细介绍

CRL-1420 MIAPaCa-2 胰腺癌细胞系

ATCC® Number: CRL-1420™  Price:  
Designations: MIA PaCa-2
Depositors: A Yunis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, single cells and loosely attached clusters
Organism: Homo sapiens (human)
Morphology: attached epithelial with floating rounded cells

Source: Organ: pancreas 
Disease: carcinoma
Cellular Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1975
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10 
D13S317: 12,13 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 12,13 
THO1: 9,10 
TPOX: 9 
vWA: 15
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
Isoenzymes: G6PD, B
Age: 65 years   CRL-1420 MIAPaCa-2 胰腺癌细胞系
Gender: male
Ethnicity: Caucasian
Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. [58034
Hybridomas have been made against the CSF-I produced by this line ATCCHB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). 
The line is sensitive to asparaginase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040  
CRL-1420 MIAPaCa-2 胰腺癌细胞系
References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
58034: Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918
CRL-1420 MIAPaCa-2 胰腺癌细胞系

MLE-12 小鼠肺上皮细胞
saos-2 人成骨肉瘤细胞
MA104 非洲绿猴胚胎肾上皮细胞
ball-1 细胞
nci-h226 人肺鳞癌细胞
MCA38 小鼠结肠癌细胞
LTPA 小鼠胰腺癌细胞 
NCI-H23 人非小细胞肺癌细胞
293.00     人胚肾细胞

CRL-1420 MIAPaCa-2 胰腺癌细胞系 
293FT    人胚肾细胞-用于慢病毒包装
293TN    人胚肾细胞--用于慢病毒包装
293AV    人胚肾细胞-用于腺病毒包装
293T    人胚肾细胞
208F    大鼠成纤维细胞
786-O    人肾透明细胞腺癌细胞
A172    人脑胶质母细胞瘤细胞
A549    人肺癌细胞 细胞系购买
AGS    人胃癌细胞
BEL7402 人肝癌细胞系
AZ-521    人胃癌细胞
B16F0    小鼠黑色素瘤细胞
Bel-7402    人肝癌细胞
Bel-7404    人肝癌细胞
BGC-823    人胃癌细胞
C6    大鼠脑胶质瘤细胞
SW480    人结肠腺癌细胞
CaCo-2    人结肠癌细胞
Cho-K1    仓鼠卵巢细胞亚株
COS-7    非洲绿猴SV40转化的肾细胞
GES-1    人胃上皮细胞
HCT-8    人回盲肠癌细胞
HeLa    人宫颈癌细胞
Hepa1-6    小鼠肝癌细胞
HepG2    人肝癌细胞
hFOB1.19    人永生化成骨细胞
HGC-27    人胃癌细胞
Hs 746T    人胃癌细胞
HT-29    人结肠癌细胞
KB    人口腔表皮样癌细胞
LoVo    人结肠癌细胞
LLC-PK1    猪肾近曲小管上皮细胞
MCF-10A    人正常乳腺上皮细胞
MCF-7    人乳腺癌细胞
MDA-MB-231    人乳腺癌细胞
MGC80-3    人胃癌细胞
MKN-45    人胃癌细胞
NCI-H460    人大细胞肺癌细胞
NIH-3T3    小鼠成纤维细胞
NUGC-3    人胃癌细胞
Raw264-7    小鼠单核细胞白血病细胞
SGC-7901    人胃癌细胞
SMMC-7721    人肝癌细胞
SNU-638    人胃癌细胞
SNU-739    人肝癌细胞
Tca8113    人舌鳞癌细胞    细胞系购买
U251    人脑胶质瘤细胞
U-373MG    人脑神经胶质瘤细胞
U-87 MG    人脑星形胶质母细胞瘤
BT-474    人乳腺导管癌细胞
PANC-1    人胰腺癌细胞
HEK293    人胚肾细胞
Hep3B    人肝癌细胞
HL-7702    人正常肝细胞
Raw-Blue    小鼠单核细胞白血病细胞
WEHI-231    小鼠B淋巴细胞
OPM-2    人骨髓瘤细胞
NCI-H929    人骨髓瘤细胞

CRL-1420 MIAPaCa-2 胰腺癌细胞系 
U266    人骨髓瘤细胞
EJ    人膀胱癌细胞
HOC    大鼠肝卵圆细胞
RWPE-1    人正常前列腺上皮细胞
CAL 27    人舌鳞癌细胞
Calu-3    人肺腺癌细胞
Eca-109    人食管癌细胞
DLD-1    人结直肠腺癌上皮细胞
5637.00     人膀胱癌细胞
SK-OV-3    人卵巢癌细胞
GBC-SD    人胆囊癌细胞
BEL7402 人肝癌细胞系
SW620    人结肠癌细胞
MRC-5    人胚肺成纤维细胞     细胞系购买
PC9    人肺癌细胞
PC14    人肺癌细胞
BxPC    人原位胰腺腺癌细胞
PK(15)    猪肾上皮细胞
MDCK    狗肾上皮细胞
3D4/21    猪肺泡巨噬细胞
     CRL-1420 MIAPaCa-2 胰腺癌细胞系 
    
    
MHCC-97L    人肝癌细胞(低转移)
MHCC-97H    人肝癌细胞系(高转移)
MiaCaPa-2    人胰腺癌细胞
HN13    口腔鳞状细胞癌细胞
Bend3    小鼠脑微血管内皮细胞
QBC939    人胆管癌细胞
Ishikawa    人子宫内膜癌细胞
A498    人肾癌细胞
143B    
WEHI-231    小鼠B淋巴细胞
BN    
P9R    小鼠骨髓基质细胞
P9    小鼠骨髓基质细胞
F98    大鼠脑胶质瘤细胞
NCIH1299    人非小细胞肺癌细胞
NCIH1650-M3    人非小细胞肺癌细胞
293LVT    人胚肾细胞--用于慢病毒包装
EC9706    人食管癌细胞
CNE    人鼻咽癌细胞
H322    人非小细胞肺癌细胞
Panc O2    
S8    BEL7402 人肝癌细胞系
R5    细胞系购买
Pig MSC    猪骨髓间质干细胞
HCAEC    人冠状动脉内皮细胞
marc-145    猴胚胎肾上皮细胞
3D4/21    猪肺泡巨噬细胞
PK(15)    猪肾上皮细胞
MADB-106    大鼠乳腺癌细胞
Sf9    昆虫卵巢细胞
DADY    
HUVEC[HUV-EC-C]    人脐静脉内皮细胞
PC14    人肺癌细胞
PC9    人肺癌细胞
Eca-109    人食管癌细胞
CAL 27    人舌鳞癌细胞
HOC    肝卵圆细胞
EJ    人膀胱癌细胞

HGC-27 人胃癌细胞(未分化
U266    人骨髓瘤细胞
293-mTLR5    人胚肾细胞
Raw-Blue    小鼠单核细胞白血病细胞
HL-7702    人正常肝细胞
Hep3B    人肝癌细胞
U-373MG    人脑神经胶质瘤细胞
Tca-8113    人舌鳞癌细胞
SNU-739    人肝癌细胞
SNU-638    人胃癌细胞
NUGC-3    人胃癌细胞
NIH-3T3    小鼠成纤维细胞
MKN-45    人胃癌细胞
LLC-PK1    猪肾近曲小管上皮细胞
Hs 746T    人胃癌细胞
Hepa1-6    小鼠肝癌细胞
HCT-8    人回盲肠癌细胞
BEL7402 人肝癌细胞系
HaCaT    人永生化表皮细胞
GES-1    人胃上皮细胞
B16-F0    小鼠黑色素瘤细胞
AZ-521    人胃癌细胞
786-O    人肾透明细胞腺癌细胞
208F    大鼠成纤维细胞
293TN    人胚肾细胞--用于慢病毒包装
293FT    人胚肾细胞-用于慢病毒包装
GP-293    人胚肾细胞
293-L.P.    人胚肾细胞

CRL-1420 MIAPaCa-2 胰腺癌细胞系