CRL-1840 OK 负鼠肾细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和优培养条件!

ATCC® Number: CRL-1840™
Designations: OK
Depositors: MM Miller
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Didelphis marsupialis virginiana (opossum)
Morphology: epithelial

Source: Organ: kidney, cortex
Tissue: proximal tubule
Disease: normal
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: alpha 2 adrenergic, expressed
atrial natriuretic peptide (ANP), expressed
serotonin, expressed
parathyroid hormone (PTH), expressed
alpha 2 adrenergic; serotonin; parathyroid hormone (PTH); atrial natriuretic peptide (ANP)
Age: adult
Gender: female
Comments: This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epithelium.
The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References:
1601: Cole JA, et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3', 5'-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-2989, 1988. PubMed:
1602: Nakai M, et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem. Biophys. Res. Commun.
22318: Cheng L, et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem. Biophys. Res. Commun.
22651: Koyama H, et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-246, 1978. PubMed: 566717
23383: . . J. Pharmacol. 244: 571-578, 1988.