Designations: |
EL4.IL-2 |
Depositors: |
JJ Farrar |
Biosafety Level: |
1 |
Shipped: |
frozen |
Medium & Serum: |
See Propagation |
Growth Properties: |
suspension |
Organism: |
Mus musculus (mouse) |
Morphology: |
lymphoblast
|
Source: |
Organ: thymus
Strain: C57BL/6
Disease: lymphoma |
Cellular Products: |
interleukin-2 (interleukin 2, IL-2) |
Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
Comments: |
This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).
The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.
Tested and found negative for ectromelia virus (mousepox). |
Propagation: |
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C |