Designations: |
AE-1 |
Depositors: |
DM Fambrough |
Isotype: |
IgG1 |
Biosafety Level: |
1 |
Shipped: |
frozen |
Medium & Serum: |
See Propagation |
Growth Properties: |
suspension |
Organism: |
Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma)) |
Morphology: |
lymphoblast
|
Source: |
Cell Type: hybridoma: B lymphocyte; |
Cellular Products: |
immunoglobulin; monoclonal antibody; against human acetylcholinesterase |
Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
Comments: |
Animals were immunized with purified human erythrocyte acetylcholinesterase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to human and mon key acetylcholinesterase, but reacts with a different epitope than AE-2 (ATCC HB-73).
Tested and found negative for ectromelia virus (mousepox). |
Propagation: |
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |