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D341 Med 人髓母细胞瘤

ATCC® Number:	HTB-187™	Price:	$323.00
Designations: D341 Med Depositors: HS Friedman Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Homo sapiens (human) Morphology: spheroid Source: Organ: brain Tissue: cerebellum Disease: medulloblastoma Cellular Products: neurofilament proteins Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes DNA Profile (STR): Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,13 D16S539: 12,14 D5S818: 11,12 D7S820: 9,13 F13A01: 6,7 F13B: 8,10 FESFPS: 10,13 LPL: 11 THO1: 6,9.3 TPOX: 8,11 vWA: 17,18 Cytogenetic Analysis: This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy. Age: 3.5 years Gender: male Comments: The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. The cells produce tumors in nude mice. The cell line is positive forexpressionof neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. The c-myc oncogene is amplified in D341 Med cells. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: 2 to 3 times per week Preservation: Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase

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D341 Med 人髓母细胞瘤
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D341 Med 人髓母细胞瘤
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ROS1728 大鼠成骨细胞
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HTB-58 SK-MES-1 人肺鳞癌细胞
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BALL-1 人B淋巴细胞急性白血病细胞
D341 Med 人髓母细胞瘤
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BS-C-1 非洲绿猴肾细胞
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3AO人卵巢癌细胞
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u87MG 人脑星形胶质母细胞瘤
Renca 小鼠肾癌细胞
nci-h929 骨髓瘤细胞
P388小鼠白血病细胞
D341 Med 人髓母细胞瘤
opm-2 骨髓瘤细胞
MDA-MB-435 人乳腺癌高转移细胞
KU812 人外周血嗜碱性白细胞
NIH:OVCAR-3 人卵巢癌细胞
SW 1990人胰腺癌细胞系
S180人结肠腺癌细胞
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NCI-H446 人小细胞肺癌细胞
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RKO 人结肠腺癌细胞
293A 人胚肾细胞
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