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This line has a complex hyperdiploid karyotype of 66XX to 88XX.
Age:
47 years
Gender:
female
Ethnicity:
Black
Comments:
The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman.
The tumor was described as a poorly differentiated ovarian clear cell carcinoma.
Initially, the cells were grown in soft agar.
The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).
ES-2 cells express low levels of P glycoprotein.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C