| 产品名称： |
Jurkat, Clone E6-1 人T淋巴细胞白血病细胞 |
| 产品型号： |
Jurkat, Clone E6-1 |
| 品牌： |
995 |
| 产品数量： |
|
| 产品单价： |
面议 |
| 日期: |
2024-09-04 |
Jurkat, Clone E6-1 人T淋巴细胞白血病细胞的详细资料
Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
TIB-152 Jurkat, Clone E6-1 人T淋巴细胞白血病细胞 的详细介绍
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ATCC® Number:
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TIB-152™
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Price:
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Designations:
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Jurkat, Clone E6-1
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Depositors:
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A Weiss
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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suspension
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Organism:
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Homo sapiens
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Morphology:
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Source:
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Disease: acute T cell leukemia
Cell Type: T lymphocyte;
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Cellular Products:
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interleukin-2 (interleukin 2, IL-2)
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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TIB-152 Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
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Applications:
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transfection host
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Receptors:
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T cell antigen receptor, expressed
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Antigenexpression:
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CD3; Homo sapiens, expressed
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DNA Profile (STR):
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Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,10
vWA: 18
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Cytogenetic Analysis:
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This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%. The karyotype is 46,XY,-2,-18,del(2) (p21p23),del(18) (p11.2). Most cells had normal X and Y chromosomes.
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Gender:
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male
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Comments:
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This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production.
The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free.
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Subculturing:
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Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell density to exceed 3 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentraion between between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
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Preservation:
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Doubling Time:
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48 hrs TIB-152 Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1990
derivative:ATCC CRL-2063
derivative:ATCC TIB-153
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References:
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1609: Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133: 123-128, 1984. PubMed: 6327821
23430: Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951
32253: Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127
32368: Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNAexpressionin primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194
32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
32446: Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663
32561: Tiffany HL, et al. Enhancedexpressionof the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842
32704: Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984
32755: Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695
32796: Bloom TJ, Beavo JA. Identification and tissue-specificexpressionof PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082
32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
32904: Linette GP, et al. Cross talk between cell death and cell cycle progression: BCL-2 regulates NFAT-mediated activation. Proc. Natl. Acad. Sci. USA 93: 9545-9552, 1996. PubMed: 8790367
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538
32922: Yang RY, et al.expressionof galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. USA 93: 6737-6742, 1996. PubMed: 8692888
33013: Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141
33025: Ponton A, et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J. Biol. Chem. 271: 8991-8995, 1996. PubMed: 8621545
33042: August A, Dupont B. Association between mitogen-activated protein kinase and the zeta chain of the T cell receptor (TcR) with the SH2,3 domain of p56lck. J. Biol. Chem. 271: 10054-10059, 1996. PubMed: 8626561
33122: Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328
33136: Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998
33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
50685: Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013
112530: Ronald Wange, personal communication
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Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528
小鼠胰腺癌细胞 LTPA CRL-2389
大鼠胰腺癌细胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴细胞 SP2/MIL-6 CRL-2016
大鼠脑间质细胞 DI TNC1 CRL-2005
小鼠肌原细胞 C2C12 CRL-1772
鹌鹑纤维肉瘤细胞 QT6 CRL-1708
大鼠回肠细胞 IEC-18 CRL-1589
非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纤维细胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮细胞 WPE-int CRL-2888
人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854
人胰腺导管腺癌细胞 PL45 CRL-2558
人胰腺癌细胞 Panc 05.04 CRL-2557
人胰腺癌细胞 Panc 04.03 CRL-2555
人胰腺癌细胞 Panc 02.03 CRL-2553
人胰腺癌细胞 Panc 08.13 CRL-2551
人胰腺癌细胞 Panc 03.27 CRL-2549
人胰腺癌细胞 Panc 10.05 CRL-2547
人肝癌细胞 SNU-398 CRL-2233
人成神经瘤细胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌细胞 HPAC CRL-2119
人胰腺癌细胞 HPAF-II CRL-1997
人黑色素瘤细胞 A375.S2 CRL-1872
人脐静脉内皮细胞 HUV-EC-C CRL-1730
人黑素瘤细胞 WM-266-4 CRL-1676
人膀胱癌细胞 HT-1376 CRL-1472
人正常前列腺上皮细胞 RWPE-2 CRL-11610
人正常前列腺上皮细胞 RWPE-1 CRL-11609
鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203
人乳腺癌细胞 MDA-MB-231 HTB-26
Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
小鼠巨噬细胞 RAW 264.7 TIB-71
人横纹肌肉瘤细胞 A-204 HTB-82
人胰腺癌细胞 Capan-2 HTB-80
人胰腺癌细胞 Capan-1 HTB-79
人乳腺导管癌细胞 MDA-MB-435S HTB-129
人胃癌细胞 Hs 746T HTB-135
人肺腺癌细胞 NCI-H441 [H441] HTB-174
人骨髓瘤细胞 U266 TIB-196
人结肠癌细胞 NCI-H716 [H716] CCL-251
人多形性恶性胶质瘤 T98G [T98-G] CRL-1690
人神经母细胞瘤细胞 BE(2)-C CRL-2268
人肾透明细胞癌细胞 Caki-2 HTB-47
人皮肤成纤维细胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤细胞系
CRL-5826 NCI-H226 人肺鳞癌细胞系
Tca-8113 人舌鳞癌细胞
MA-891 小鼠乳腺癌高转移细胞
U14 小鼠宫颈癌细胞
CRL-9518 L5178Y 小鼠淋巴瘤细胞
HTB-112 HEC-1-A 人子宫内膜腺癌细胞
HTB-113 HEC-1-B 人子宫内膜腺癌细胞
AN3 CA 人子宫内膜腺癌细胞
ROS1728 大鼠成骨细胞
CRL-6323 B16F1 小鼠黑色素瘤细胞
HTB-58 SK-MES-1 人肺鳞癌细胞
CCL-127 IMR-32 人神经母细胞瘤细胞
HTB-79 Capan-2 人胰腺癌细胞
BALL-1 人B淋巴细胞急性白血病细胞
Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
CRL-2922 Eayh926 人脐静脉细胞融合细胞
WB-F344 大鼠肝干细胞
CRL-1715 TM4 正常小鼠睾丸细胞
CRL-2539 4T1 小鼠乳腺癌细胞
CRL-1442 BRL-3A 大鼠肝细胞
BRL 大鼠肝细胞
RSC96大鼠雪旺细胞
Isk 人子宫内膜癌细胞
HTB-113 hec-1-b 人子宫内膜腺癌细胞
PLA-802?人恶性横纹肌肉瘤
Pt-K2 袋鼠肾细胞
BS-C-1 非洲绿猴肾细胞
A498人肾癌细胞
3AO人卵巢癌细胞
iec-6 大鼠小肠隐窝上皮细胞
u87MG 人脑星形胶质母细胞瘤
Renca 小鼠肾癌细胞
nci-h929 骨髓瘤细胞
P388小鼠白血病细胞
opm-2 骨髓瘤细胞
MDA-MB-435 人乳腺癌高转移细胞
KU812 人外周血嗜碱性白细胞
NIH:OVCAR-3 人卵巢癌细胞
SW 1990人胰腺癌细胞系
S180人结肠腺癌细胞
HuTu-80十二指肠腺癌
RL95-2 人子宫内腺癌细胞
NCI-H446 人小细胞肺癌细胞
MSTO-211H 人肺癌细胞
H4-Ⅱe 大鼠肝癌细胞系
HLF 人肺成纤维样细胞
LS 180人结肠腺癌细胞
HuTu-80十一指肠腺癌
ST猪睾丸细胞系
REH人急性非B非T淋巴细胞白血病
NCI-H520 人肺鳞癌细胞
RKO 人结肠腺癌细胞
293A 人胚肾细胞
RK-13 兔肾细胞系
