| 产品名称： |
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞 |
| 产品型号： |
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| 品牌： |
995 |
| 产品数量： |
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| 产品单价： |
面议 |
| 日期: |
2024-09-04 |
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞的详细资料
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞 的详细介绍
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
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ATCC® Number:
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CRL-1973™
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Price:
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Designations:
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NTERA-2 cl.D1 [NT2/D1]
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Depositors:
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PW Andrews
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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adherent
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Organism:
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Homo sapiens (human)
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Morphology:
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epithelial-like,differentiation changes phenotype
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Source:
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Organ: testis
Disease: malignant pluripotent embryonal carcinoma
Derived from metastatic site: lung
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
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Applications:
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transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
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Virus Susceptibility:
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RA and HMBA TREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
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Virus Resistance:
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UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
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DNA Profile (STR):
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Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12,13
D5S818: 9,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 18,19
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Cytogenetic Analysis:
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This is a hypotriploid human cell line with the modal chromosome number of 63 in 48% of cells examined. However, cells with 62 chromosome counts also occurred at a rather high frequency (24%). The rate of polyploidy was 1.6%.,about12 marker chromosomes are constantly found in most cells. They include: der(9)t(1;9)(q25;q34.3); del(1)(q25); der(13)t(11;13)(q13;q34); t(Xq1q); and eight others., At least two markers are found only in some cells. The normal Y chromosome was found in all cells. only single copies of normal chromosomes 1, 10, 11 and 13 were present. Others were mostly in two or three copies per cell.
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Age:
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22 years CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
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Gender:
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male
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Ethnicity:
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Caucasian
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Comments:
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The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. [22741]
The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106).
This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide ((HMBA). [22321] [22999]
The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters.
The cells exhibit highexpressionof N-myc oncogene activity.
To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid.
Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere).
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Subculturing:
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Medium Renewal: Every 2 to 3 days
Subcultures are prepared by scraping.
Cells from confluent cultures (approximately 20 million cells per 75 sq. cm.) are dislodged from the flask surface, aspirated and dispensed into new flasks.
Cultures should be maintained at high density.
Seed new flasks at a density of at least 5 X 10 exp6 viable cells per 75 sq. cm. flask.
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Preservation:
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culture medium 95%; DMSO, 5%
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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References:
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22321: Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662
22336: Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410
22407: Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853
22741: Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356
22746: Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286
22947: Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309
22999: Andrews PW. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603
23235: Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047
29000: Dewji NN, Singer SJ. Cell surfaceexpressionof the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228
49353: Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenouscriptOexpressionand inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871
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iec-6 大鼠小肠隐窝上皮细胞
马-达二氏犬肾细胞系 MDCK (NBL-2) CRL-34
鸭子胚胎成纤维细胞 Duck embryo CCL-141
小鼠胚胎成纤维细胞 3T3-L1 CCL-173
人结肠直肠腺癌 COLO 320DM CCL-220
人结肠直肠腺癌 NCI-H508 CRL-253
人结肠直肠腺癌 COLO 320HSR CCL-220.1
人B淋巴细胞 Ramos（RA1） CRL-1596
人淋巴母细胞 T2(174×CEM.T2) CRL-1992
大鼠心肌细胞 H9C2(2-1) CRL-1446
猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528
小鼠胰腺癌细胞 LTPA CRL-2389
大鼠胰腺癌细胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴细胞 SP2/MIL-6 CRL-2016
大鼠脑间质细胞 DI TNC1 CRL-2005
小鼠肌原细胞 C2C12 CRL-1772
鹌鹑纤维肉瘤细胞 QT6 CRL-1708
大鼠回肠细胞 IEC-18 CRL-1589
非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纤维细胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮细胞 WPE-int CRL-2888
人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854
人胰腺导管腺癌细胞 PL45 CRL-2558
人胰腺癌细胞 Panc 05.04 CRL-2557
人胰腺癌细胞 Panc 04.03 CRL-2555
人胰腺癌细胞 Panc 02.03 CRL-2553
人胰腺癌细胞 Panc 08.13 CRL-2551
人胰腺癌细胞 Panc 03.27 CRL-2549
人胰腺癌细胞 Panc 10.05 CRL-2547
人肝癌细胞 SNU-398 CRL-2233
人成神经瘤细胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌细胞 HPAC CRL-2119
人胰腺癌细胞 HPAF-II CRL-1997
人黑色素瘤细胞 A375.S2 CRL-1872
人脐静脉内皮细胞 HUV-EC-C CRL-1730
人黑素瘤细胞 WM-266-4 CRL-1676
人膀胱癌细胞 HT-1376 CRL-1472
人正常前列腺上皮细胞 RWPE-2 CRL-11610
人正常前列腺上皮细胞 RWPE-1 CRL-11609
鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203
人乳腺癌细胞 MDA-MB-231 HTB-26
小鼠巨噬细胞 RAW 264.7 TIB-71
人横纹肌肉瘤细胞 A-204 HTB-82
人胰腺癌细胞 Capan-2 HTB-80
人胰腺癌细胞 Capan-1 HTB-79
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
人乳腺导管癌细胞 MDA-MB-435S HTB-129
人胃癌细胞 Hs 746T HTB-135
人肺腺癌细胞 NCI-H441 [H441] HTB-174
人骨髓瘤细胞 U266 TIB-196
人结肠癌细胞 NCI-H716 [H716] CCL-251
人多形性恶性胶质瘤 T98G [T98-G] CRL-1690
人神经母细胞瘤细胞 BE(2)-C CRL-2268
人肾透明细胞癌细胞 Caki-2 HTB-47
人皮肤成纤维细胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤细胞系
CRL-5826 NCI-H226 人肺鳞癌细胞系
Tca-8113 人舌鳞癌细胞
MA-891 小鼠乳腺癌高转移细胞
U14 小鼠宫颈癌细胞
CRL-9518 L5178Y 小鼠淋巴瘤细胞
HTB-112 HEC-1-A 人子宫内膜腺癌细胞
HTB-113 HEC-1-B 人子宫内膜腺癌细胞
AN3 CA 人子宫内膜腺癌细胞
ROS1728 大鼠成骨细胞
CRL-6323 B16F1 小鼠黑色素瘤细胞
HTB-58 SK-MES-1 人肺鳞癌细胞
CCL-127 IMR-32 人神经母细胞瘤细胞
HTB-79 Capan-2 人胰腺癌细胞
BALL-1 人B淋巴细胞急性白血病细胞
CRL-2922 Eayh926 人脐静脉细胞融合细胞
WB-F344 大鼠肝干细胞
CRL-1715 TM4 正常小鼠睾丸细胞
CRL-2539 4T1 小鼠乳腺癌细胞
CRL-1442 BRL-3A 大鼠肝细胞
BRL 大鼠肝细胞
RSC96大鼠雪旺细胞
Isk 人子宫内膜癌细胞
HTB-113 hec-1-b 人子宫内膜腺癌细胞
PLA-802?人恶性横纹肌肉瘤
Pt-K2 袋鼠肾细胞
BS-C-1 非洲绿猴肾细胞
A498人肾癌细胞
3AO人卵巢癌细胞
iec-6 大鼠小肠隐窝上皮细胞
u87MG 人脑星形胶质母细胞瘤
Renca 小鼠肾癌细胞
nci-h929 骨髓瘤细胞
P388小鼠白血病细胞
opm-2 骨髓瘤细胞
MDA-MB-435 人乳腺癌高转移细胞
KU812 人外周血嗜碱性白细胞
NIH:OVCAR-3 人卵巢癌细胞
SW 1990人胰腺癌细胞系
S180人结肠腺癌细胞
HuTu-80十二指肠腺癌
RL95-2 人子宫内腺癌细胞
NCI-H446 人小细胞肺癌细胞
MSTO-211H 人肺癌细胞
H4-Ⅱe 大鼠肝癌细胞系
HLF 人肺成纤维样细胞
LS 180人结肠腺癌细胞
HuTu-80十一指肠腺癌
ST猪睾丸细胞系
REH人急性非B非T淋巴细胞白血病
NCI-H520 人肺鳞癌细胞
RKO 人结肠腺癌细胞
293A 人胚肾细胞
RK-13 兔肾细胞系
CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
