Organ: brain Disease: neuroepithelioma Derived from metastatic site: supra-orbital area
Permits/Forms:
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HTB-10 SK-N-MC 人神经上皮瘤细胞
Restrictions:
The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line. [26318]
This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler.SK-N-MC was isolated in September of l971 and was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
